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Laurent Colot (1520–1590) was a French people physician.
Colot specialized in the treatment of , using secret techniques he inherited from his father Germain Colot. Laurent was named lithotomy of the Hôtel-Dieu in Paris and, in 1556, was named Operator of Stones for Henri II, although Ambroise Paré was the king's general surgeon.
Colot was the first of several generations of Paris lithotomists.
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